USE OF CONJUGATIVE AND THERMOSENSITIVE CLONING VECTORS FOR TRANSPOSONDELIVERY TO MYCOBACTERIUM-SMEGMATIS

Authors
GAVIGAN JA GUILHOT C GICQUEL B MARTIN C
Citation
Ja. Gavigan et al., USE OF CONJUGATIVE AND THERMOSENSITIVE CLONING VECTORS FOR TRANSPOSONDELIVERY TO MYCOBACTERIUM-SMEGMATIS, FEMS microbiology letters, 127(1-2), 1995, pp. 35-39
Citations number
11
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
127
Issue
1-2
Year of publication
1995
Pages
35 - 39
Database
ISI
SICI code
0378-1097(1995)127:1-2<35:UOCATC>2.0.ZU;2-8
Abstract
Conjugative, thermosensitive shuttle plasmids capable of transfer from Escherichia coli to Mycobacterium smegmatis were constructed. They co ntain both an E. coli replicon and a thermosensitive derivative of the pAL5000 mycobacterial replicon. Using a temperature shift protocol, t he conjugative plasmid, pJAZ11 was used to deliver the transposon Tn61 1 from E. coli into the chromosome of M. smegmatis. Analysis of transc onjugants revealed the random insertion of the transposon.