A SEROTYPE-SPECIFIC EPITOPE OF DENGUE-VIRUS-1 IDENTIFIED BY PHAGE DISPLAYED RANDOM PEPTIDE LIBRARY

From a panel of monoclonal antibodies of dengue viruses, a serotype-sp ecific epitope of dengue virus 1 was screened from a random peptide li brary displayed on phage. The epitope was the determinant reactive wit h monoclonal antibody 15F3-1 that was specific to dengue 1. The screen ing was monitored by a dot blotting procedure, and after three rounds of screening a consensus motif, HRYSWK, was found. This sequence match es the sequence HKYSWK, corresponding to the amino acid residues 885-8 90 of polyprotein or residues 111-116 of the non-structural protein 1 of dengue virus serotype 1. The linear epitope was confirmed by testin g the antigenicity of chemically synthesized 8-branched peptide.