CHARACTERIZATION OF EUKARYOTIC-LIKE KINASE-ACTIVITY IN ESCHERICHIA-COLI USING THE GENE-PROTEIN DATABASE

Authors
SWEENEY ST FREESTONE P NORRIS V
Citation
St. Sweeney et al., CHARACTERIZATION OF EUKARYOTIC-LIKE KINASE-ACTIVITY IN ESCHERICHIA-COLI USING THE GENE-PROTEIN DATABASE, FEMS microbiology letters, 127(1-2), 1995, pp. 133-138
Citations number
19
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
127
Issue
1-2
Year of publication
1995
Pages
133 - 138
Database
ISI
SICI code
0378-1097(1995)127:1-2<133:COEKIE>2.0.ZU;2-B
Abstract
The gene-protein database was used to obtain the two-dimensional polya crylamide gel coordinates of proteins phosphorylated in extracts of Es cherichia coli including those phosphorylated by eukaryotic-like kinas e activities. These suggest that the phosphoproteins correspond to, or co-migrate with, the product of an open reading frame at 1.3 min (Orf 80), Enzyme 1 of the phosphoenolpyruvate-dependent phosphotransferase system (PtsI), the tRNA synthetase for histidine (HisS), and proteins involved in the response to carbon starvation and quinone treatment.