M. Awamura et al., IN-VITRO GERMINATION OF CAPRIFIG POLLEN G RAINS USING STIGMATIC EXUDATE OF A COMMON FIG AS A PROMOTER, Engei Gakkai Zasshi, 63(4), 1995, pp. 739-743
To improve the pollen germination of caprifig 'VC-180' (Ficus carica L
. var. intermedia Shinn X F. carica L. var. sylvestris Shinn) in vitro
, stigmatic exudate from long-styled pistillate flowers of 'Masui Daup
hine' (F. carica L. var. hortensis Shinn) was added to a medium. 1. Th
e optimum pollen germination of 'VC-180' occurred when 2 or 3 pistilla
te flowers of 'Masui Dauphine' were embedded per cm(2) of basal medium
(1% agar+10% sucrose) for 3 hours under 5 degrees to 15 degrees C. 2.
More than 70% of the pollen grains germinated and grew vigorously dur
ing a 7-hour-culture kept at 25 degrees to 30 degrees C. 3. Pistillate
flowers within a syconium (20 similar to 32 mm diam.) of the second c
rop of 'Masui Dauphine' figs promoted pollen germination. Likewise, po
llen grains from the summer crop of caprifig 'VC-180' germinated regar
dless of the maturity of the syconium in the presence of the stigmatic
exudate.