EFFECTS OF IONIC-STRENGTH AND PH ON THE ADSORPTION EQUILIBRIA OF LYSOZYME ON ION-EXCHANGERS

The effects of ionic strength and pH on adsorption of lysozyme to thre e strong cation exchangers have been studied. Adsorption equilibrium d ata obtained using the batch techniques corresponded well to the Langm uir isotherm. Ionic strength had a considerable influence on the isoth erms. In all cases, the maximum binding capacity of the three exchange rs decreased while the apparent dissociation constant increased with i ncreasing ionic strength. The maximum binding capacity also decreased while the apparent dissociation constant was not significantly affecte d by an increase in pH. It was found that the three exchangers exhibit ed different levels of binding capacity under identical solution condi tions. This was shown to be caused by variation in the arrangement and distribution of charged groups. However, the ion-exchange matrix had little effect on the apparent dissociation constant.