JEJUNAL MUCOSAL FUNCTION OF THE ISOLATED BOWEL SEGMENT CREATED BY OMENTOENTEROPEXY IN DOGS - A STUDY BY IN-SITU LUMINAL PERFUSION

An isolated bower segment (IBS) was created in dogs by omentoenteropex y, using staged procedures. (1) Omentoenteropexy was performed at the antimesenteric border of a 15-cm jejunal segment, which was exterioriz ed at both ends (IBSB). (2) After 6 weeks, once dual vascularization t o the IBS had been established, its mesentery was divided (IBSA) or lo ngitudinally split, thus achieving its elongation (IBSE). A control do g underwent a Thiry-Vella (T-V) loop procedure of an identical jejunal segment. Viability of the IBSB and IBSA was previously proven by the authors, through angiographic studies. In the present study, the absor ption capability of IDS variants was assessed using in situ luminal pe rfusion, with a bicarbonate buffer containing glucose and labeled glyc ine. Jejunal transport rates of these solutes were calculated from the differences in their concentrations in the perfusion solution and in the effluent. Comparisons were made among the IBS variants and between them and the T-V loop. No significant difference in the absorption ca pability of glucose and glycine was noted between the various IBS vari ants. There was a marked reduction of glucose absorption and a moderat e reduction of glycine absorption in all IBS variants when compared wi th the fresh T-V loop, most probably because of disuse atrophy of the mucosa. In conclusion, absorption of glucose and glycine is preserved in the IBS, created by omentoenteropexy, both after its mesenteric div ision and following the IBS elongating procedure. Copyright (C) 1995 b y W.B. Saunders Company