L. Sikurova et R. Frankova, THE EFFECT OF ALBUMIN ON INCORPORATION OF MEROCYANINE-540 INTO PHOSPHOLIPID LIPOSOMES, General physiology and biophysics, 13(5), 1994, pp. 393-403
Absorption and fluorescence emission spectroscopy was applied to study
the changes in albumin modified incorporation of merocyanine 540 into
liposomes composed of different lecithins (DMPC, DPPC, POPC, and egg
PC). Our results confirmed high affinity of merocyanine molecules towa
rd albumin and revealed that albumin competed with all phospholipids u
sed for binding merocyanine 540 molecules. However, the extent of this
competition was determined by the kind of phospholipid. Albumin compe
ted very successfully with lecithins containing saturated fatty acid c
hains (DPPC, DMPC) and weakly with unsaturated lecithins (POPC, egg PC
) for binding merocyanine 540 molecules.