EVIDENCE OF A SELECTIVE DEPLETION OF A CD16(-KILLER-CELL SUBSET DURING HIV-INFECTION() CD56(+) CD8(+) NATURAL)

Three-color flow cytometric analysis of CD16+ natural killer (NK) cell s was assessed in HIV seropositive patients and healthy heterosexual c ontrols. A selective depletion of lymphocytes with the CD16+ NK phenot ype was found among the HIV+ infected patients. When the CD16 lymphocy te subset was further evaluated by three-color flow cytometry, cells b earing both the CD8 and CD56 antigens were significantly decreased. An alysis of activation antigens revealed a large proportion of CD16(+) N K cells from HIV+ patients expressed HLA-DR, but this did not correlat e with CD25 (IL-2 receptor) expression, The overall loss of the CD8 an d CD56 antigens among the NK population with an increase in activation status may be due to differential loss of the NK cell subsets or, alt ernatively, to the loss of immunoregulatory cytokines, which have been shown to be important in maintaining NK activity. Whether these chang es in the NK compartment may influence the outcome of individuals with HIV disease still remains an open question but is an important issue when performing phenotypic analysis of HIV+ subjects. (C) 1995 Wiley-L iss, Inc.