CLONING AND CHARACTERIZATION OF MULTIPLE ACETYL-COA CARBOXYLASE TRANSCRIPTS IN OVINE ADIPOSE-TISSUE

A full-length ovine acetyl-CoA carboxylase-encoding cDNA (ACC) has bee n cloned from adipose tissue and completely sequenced. The open readin g frame of 7041 nucleotides (nt) is highly homologous to the previousl y cloned human, rat, chicken, yeast and algal ACC (85, 89, 82, 54 and 54% identity, respectively). Transcript heterogeneity was found in the 5' and 3' untranslated regions (UTR) resulting in ACC transcripts in the range of 9.0 kb to 9.4 kb. Heterogeneity at the 5' end was generat ed by the insertion of a 47-nt sequence, resulting in transcripts with either 272 or 319 nt in the 5'-UTR. Heterogeneity at the 3' end was t he result of the use of different polyadenylation signals. RNase prote ction analysis demonstrated that shorter transcripts containing 1635 n t predominated over longer transcripts of 2065 nt in the 3'-UTR.