AMINOLYSIS OF ACYL-CHYMOTRYPSINS BY AMINO-ACIDS - KINETIC APPEARANCE OF CONCENTRATION-EFFECT IN PEPTIDE YIELD ENHANCEMENT BY FREEZING

Authors
TOUGU V TALTS P MEOS H HAGA M AAVIKSAAR A
Citation
V. Tougu et al., AMINOLYSIS OF ACYL-CHYMOTRYPSINS BY AMINO-ACIDS - KINETIC APPEARANCE OF CONCENTRATION-EFFECT IN PEPTIDE YIELD ENHANCEMENT BY FREEZING, Biochimica et biophysica acta. Protein structure and molecular enzymology, 1247(2), 1995, pp. 272-276
Citations number
18
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta. Protein structure and molecular enzymology → ACNP
ISSN journal
01674838
Volume
1247
Issue
2
Year of publication
1995
Pages
272 - 276
Database
ISI
SICI code
0167-4838(1995)1247:2<272:AOABA->2.0.ZU;2-M
Abstract
The effects of reagent concentrations, various added substances, pH an d temperature on the yield of peptide synthesis by chymotrypsin in fro zen and liquid solutions at subzero temperatures have been studied. In creased nucleophile concentration in the liquid microinclusions of ice has been shown to be sufficient for explaining the peptide yield impr ovement found at freezing conditions.