Sm. Katz et al., IMPROVED SMALL-INTESTINAL PRESERVATION AFTER LAZAROID U74389G TREATMENT AND COLD-STORAGE IN UNIVERSITY-OF-WISCONSIN SOLUTION, Transplantation, 59(5), 1995, pp. 694-698
The small intestine (SI) is highly sensitive to oxygen free radical-in
duced injury. The most common preservation solution, University of Wis
consin (UW) solution, does not adequately prevent free radical-induced
injury, Lazaroids, and U743896 in particular, are a new class of comp
ound that are potent inhibitors of superoxide-mediated lipid peroxidat
ion. We studied the added influence of U74389G to 18-hr cold preservat
ion of rat SI in UW solution. Three groups of rats were studied. In gr
oup 1, SI was excised and reperfused immediately, In group 2, SI was s
tored in UW solution at 4 degrees C for 18 hr. In group 3, U74389G was
given to the SI graft before storage and again before reperfusion, Bl
ood reperfusion of the grafts was achieved via connection to the super
ior mesenteric artery and portal vein of support rats. Functional reco
very was assessed using a maltose tolerance test. Weight changes were
calculated and histologic studies done. After 30 and 60 min of reperfu
sion, maltose uptake in group 3 was significantly better than that of
the group 2, and returned to control levels. Significantly more tissue
swelling was noted in group 3 over control, but the magnitude was les
s than that of group 2. Less transmural necrosis and villous blunting
were noted in group 3 versus group 2; the appearance of the mucosa in
group 3 approached that of group 1. We conclude that the use of U74389
G treatment in addition to cold storage in UW solution improves recove
ry of graft function and minimizes morphologic damage to the small int
estinal mucosa.