CLONING OF ESCHERICHIA-COLI PENICILLIN-G ACYLASE GENE WITH MINI-MU CONTAINING A PLASMID REPLICON

Authors
LACZAOVA A PECHAN T STUCHLIK S KORMUTAKOVA R TURNA J
Citation
A. Laczaova et al., CLONING OF ESCHERICHIA-COLI PENICILLIN-G ACYLASE GENE WITH MINI-MU CONTAINING A PLASMID REPLICON, Biotechnology letters, 17(1), 1995, pp. 13-18
Citations number
25
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
17
Issue
1
Year of publication
1995
Pages
13 - 18
Database
ISI
SICI code
0141-5492(1995)17:1<13:COEPAG>2.0.ZU;2-7
Abstract
An in vivo cloning system based on mini-Mu derivatives was used for cl oning of E. coli penicillin G acylase gene (pac). We have constructed several recombinant clones producing penicillin G acylase and some of them exhibit approximately two times higher activity than original str ains.