Pj. Houghton et Tz. Woldemariam, USE OF A POROUS GRAPHITIC CARBON COLUMN TO SEPARATE ISOMERS OF PIPERIDINO-TYPE CHROMONE ALKALOIDS, Phytochemical analysis, 6(2), 1995, pp. 85-88
A liquid chromatography procedure which rapidly separates the two isom
ers of schumannificine and N-methylschumannificine, chromone alkaloids
isoilated from Schumanniophyton magnificum (Rubiaceae), is described.
The procedure uses a porous graphitic carbon high performance liquid
chromatographic column and is compared with other chromatographic and
chemical methods which were tried unsuccessfully. The method was adapt
ed in an attempt to prepare the individual isomers of schumannificine
and N-methylschumannificine in mg amounts but interconversion of the i
somers in aqueous medium was shown to occur too rapidly for this to be
feasible.