QUANTITATIVE-DETERMINATION OF PODOPHYLLOTOXIN AND RELATED-COMPOUNDS IN PODOPHYLLUM SPECIES BY REVERSE-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
BASTOS JK KOPYCKI WJ BURANDT CL NANAYAKKARA NPD MCCHESNEY JD
Citation
Jk. Bastos et al., QUANTITATIVE-DETERMINATION OF PODOPHYLLOTOXIN AND RELATED-COMPOUNDS IN PODOPHYLLUM SPECIES BY REVERSE-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Phytochemical analysis, 6(2), 1995, pp. 101-105
Citations number
15
Categorie Soggetti
Biology,"Chemistry Analytical","Plant Sciences
Journal title
Phytochemical analysis → ACNP
ISSN journal
09580344
Volume
6
Issue
2
Year of publication
1995
Pages
101 - 105
Database
ISI
SICI code
0958-0344(1995)6:2<101:QOPARI>2.0.ZU;2-I
Abstract
A rapid sensitive and reliable reverse phase high performance liquid c hromatography method was developed using a Taxsil(R) column and gradie nt elution for the routine determination of podophyllotoxin and six re lated compounds, epipodophyllotoxin, alpha-peltatin, beta-peltatin, 4' -demethylpodophyllotoxin, podophyllotoxin-4-O-glycoside, and 1,2,3,4-d ehydradesoxypodophyllotoxin in Podophyllum extracts. The elaborated me thod gave well resolved peaks for these compounds with good detection response and linearity in the range 25-450 ng.