ESTABLISHMENT OF THE MARKER ORDER D1S252-D1S440-D1S453-D1S514-CEN-D1S442-D1S498-QTER IN RELATION TO THE CENTROMERE ON HUMAN-CHROMOSOME-1

Recent developments in genetic linkage mapping of the human genome hav e generated a large number of short tandem repeat polymorphic markers (Weissenbach et al. 1992, Gyapay et al. 1994), and eventual integratio n of these markers into a physical map is a logical progression. A num ber of Genethon microsatellite (CA repeat) markers have been provision ally localized to 1p13, but their exact position with respect to other sequences is unknown. In order to confirm the order of these markers and their position with respect to known genes within 1p13 and the cen tromere, we have isolated yeast artificial chromosomes (YACs) correspo nding to the markers and have carried out double and triple fluorescen ce in situ hybridization (FISH) studies. Knowledge of both the order o f microsatellite markers and their integration with a physical map of known genes can be an essential component in analysis of disease loci such as human cancer, where regions of chromosomes showing high levels of loss of heterozygosity need to be mapped in detail.