ISOLATION AND PURIFICATION OF DIGALACTOSYLDIACYLGLYCEROLS

A convenient method for digalactosyldiacylglycerols from been develope d using solid phase extraction. Commercial cartridges were utilized to prepare 100 mg fractions of galactolipids containing more than 97 % o f digalactosyldiacylglycerol. The purity of the fractions was monitore d by an isocratic normal phase analytical HPLC procedure, which was op timized using factorial design. The optimized system was successfully scaled up to a semi-preparative HPLC system with a capacity of about 1 0 mg/injection which was used to further purify the digalactosyldiacyl glycerol fraction.