OPTIMIZATION STRATEGIES IN MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY - ONE-PARAMETER OPTIMIZATIONS OF THE CONCENTRATION OF SODIUM DODECYL-SULFATE AND THE CONCENTRATION OF MODIFIERS (UREA AND GLUCOSE)

Computer-assisted procedures for the one-parameter optimization of the surfactant concentration and the concentration of urea or D-glucose a s modifiers in micellar electrokinetic capillary chromatography have b een developed. These procedures permit a rapid optimization of one par ameter on the basis of only two experiments. Predicted values are comp ared to empirically obtained optimum values. The influence of the modi fier concentration on the critical micelle concentration of sodium dod ecyl sulfate was experimentally determined in buffers commonly employe d in micellar electrokinetic chromatography. The alteration of retenti on factors of solutes caused by the influence of urea addition on the critical micelle concentration of sodium dodecyl sulfate was calculate d under the assumption of constant distribution coefficients and compa red to experimentally obtained values. It was demonstrated that the ad dition of urea or of D-glucose does not alter the phase ratio substant ially.