VALIDATION OF AN ANALYTICAL INSTRUMENTAL METHOD BY STANDARD ADDITION METHODOLOGY

A statistical procedure to validate an analytical methodology by stand ard addition methodology is described. The data set obtained in 3 cali bration experiments with standard solutions, standard additions, and p ortions of sample is used. The accuracy of the analytical results is c hecked by comparison of analyte contents in the different calibrations and from the recovery. Mathematical expressions to estimate the stati stical parameters are proposed. The statistical protocol has been appl ied to fluorimetric determination of molybdenum with alizarin S in veg etable tissues.