RAPID EXTRACTION AND CLEANUP FOR LIQUID-CHROMATOGRAPHIC DETERMINATIONOF DOMOIC ACID IN UNSALTED SEAFOOD

Domoic acid is the toxin responsible for incidents of amnesic shellfis h poisoning, A rapid extraction and cleanup for the liquid chromatogra phic determination of domoic acid in unsalted seafood is reported, The method uses a single-step extraction with 50% aqueous methanol and a selective cleanup and preconcentration with strong-anion exchange, sol id-phase extraction, Determination is performed by liquid chromatograp hy with ultraviolet absorbance detection, The detection limit was 20-3 0 ng/g, Recoveries of 93% were achieved from 0.2 to 20 mu g/g in musse l tissues, The method gave a precision of less than 3% for concentrati ons greater than 2 mu g/g and less than 6% at 0.2 mu g/g. A linear dyn amic range of 10(4) can be achieved. The method was successfully appli ed to a variety of seafood products, including mussels, razor clams, c rabs, and anchovies.