STRUCTURAL STUDIES OF THE O-SPECIFIC CHAIN AND A CORE HEXASACCHARIDE OF HAFNIA-ALVEI STRAIN-1192 LIPOPOLYSACCHARIDE

The structure of the O-specific side-chain and a core hexasaccharide o f the Hafnia alvei strain 1192 lipopolysaccharide has been investigate d. Methylation analysis, NMR spectroscopy, MALDI-TOF spectrometry, and various specific chemical degradations were the principal methods use d. It is concluded that the polysaccharide is composed of hexasacchari de repeating-units having the following structure which is partially O -acetylated in the 2-position of the --> 4)-alpha-D-Glc pA-(1 --> (70% ) and on different positions of the L-Rha residues (50%). [GRAPHICS] ]