The study of biofilms has indicated that bacteria behave fundamentally
differently in this habitat than in suspension. The aim of the study
reported here was to determine whether P. aeruginosa PAO1 undergoes an
y changes during attachment to an abiotic surface. A method for the st
udy of de novo protein synthesis in attached bacteria was developed. T
his was used to demonstrate that the synthesis of several proteins in
P. aeruginosa was influenced by attachment of cells. Attachment influe
nced the synthesis of at least 11 proteins during various stages after
attachment. Attachment per se was demonstrated to exert some effect o
n the regulation of certain genes. It is not clear whether this is at
the transcriptional or translational level, and what the nature of the
attachment-mediated signal is.