HUMAN FIBROBLASTS (KMST-6 RAS CELL-LINE) TRANSFORMED WITH CO-60 GAMMA-RAYS AND C-HA-RAS ONCOGENE PRODUCE A LARGE AMOUNT OF GRANULOCYTE-COLONY-STIMULATING FACTOR (G-CSF) - PRODUCTION IS ENHANCED BY CAMP, THEOPHYLLINE, AND BUTYRATE/

Authors
KAWASHIMA K JAHAN I FUSHIMI K MIHARA K NAMBA M
Citation
K. Kawashima et al., HUMAN FIBROBLASTS (KMST-6 RAS CELL-LINE) TRANSFORMED WITH CO-60 GAMMA-RAYS AND C-HA-RAS ONCOGENE PRODUCE A LARGE AMOUNT OF GRANULOCYTE-COLONY-STIMULATING FACTOR (G-CSF) - PRODUCTION IS ENHANCED BY CAMP, THEOPHYLLINE, AND BUTYRATE/, Cell structure and function, 20(1), 1995, pp. 41-45
Citations number
27
Categorie Soggetti
Cell Biology
Journal title
Cell structure and function → ACNP
ISSN journal
03867196
Volume
20
Issue
1
Year of publication
1995
Pages
41 - 45
Database
ISI
SICI code
0386-7196(1995)20:1<41:HF(RCT>2.0.ZU;2-N
Abstract
Human fibroblasts (KMST-6/RAS cell line), which was malignantly transf ormed in vitro with Co-60 gamma-rays and the c-Ha-ras oncogene, produc ed a large amount of granulocyte colony-stimulating factor (G-CSF). Th e production was greater during the logarithmic growth phase than duri ng the stationary phase. cAMP and theophylline, alone or in combinatio n, and butyrate significantly enhanced G-CSF production, but dexametha sone or 5-azacytidine did not. Enhanced production of G-CSF by these a gents was regulated at the posttranscriptional level. Neither the expr ession of the ras oncogene nor the tumorigenicity of the cells correla ted with the production of G-CSF.