H. Benhur et al., ASSESSMENT OF ESTROGEN-RECEPTOR DISTRIBUTION IN HUMAN ENDOMETRIUM BY DIRECT IMMUNOFLUORESCENCE, Acta obstetricia et gynecologica Scandinavica, 74(2), 1995, pp. 97-102
Objective, To use a direct immunofluorescence technique employing a fl
uorescein labeled anti-idiotypic antibody that recognizes the estrogen
receptor (ER) order to assess the distribution of ER in the uteri of
normal women throughout the normal menstrual cycle and of a woman expo
sed prenatally to diethylstilbestrol (DES). Subjects. Included in the
study were 25 women aged between 35 and 50 years and an amenorrheic pa
tient diagnosed as ''DES Syndrome'', Localization. Localization of ER
expression in frozen sections of uterine tissue was achieved by direct
immunofluorescence using a fluorescein labeled anti-idiotypic antibod
y that interacts with ER. Results, Analysis of the immunofluorescence
staining indicated that in the normal human endometrium the intensity
of ER staining varied according to the phase of the cycle as well as a
ccording to the cell type, On the other hand, endometrial ER evaluatio
n of the patient with DES syndrome showed minimal expression of ER and
after treatment with conjugated estrogens, endometrial biopsy reveale
d a significant increase in ER expression. Conclusions. These findings
indicate that the fluorescein labeled anti-idiotypic antibody can be
used to detect ER in normal and pathological human endometrium and to
monitor changes in ER expression in the endometrium during hormonal th
erapy