EVALUATION OF A BROTH MICRODILUTION ANTIFUNGAL SUSCEPTIBILITY TEST WITH A PH INDICATOR - COMPARISON WITH THE BROTH MACRODILUTION PROCEDURES

Authors
FOURNIER C GASPAR A BOILLOT F VILLARD J
Citation
C. Fournier et al., EVALUATION OF A BROTH MICRODILUTION ANTIFUNGAL SUSCEPTIBILITY TEST WITH A PH INDICATOR - COMPARISON WITH THE BROTH MACRODILUTION PROCEDURES, Journal of antimicrobial chemotherapy, 35(3), 1995, pp. 373-380
Citations number
24
Categorie Soggetti
Microbiology,"Pharmacology & Pharmacy
Journal title
Journal of antimicrobial chemotherapy → ACNP
ISSN journal
03057453
Volume
35
Issue
3
Year of publication
1995
Pages
373 - 380
Database
ISI
SICI code
0305-7453(1995)35:3<373:EOABMA>2.0.ZU;2-H
Abstract
A broth microdilution antifungal susceptibility test with a pH indicat or (bromocresol purple) in a synthetic medium was evaluated. This meth od measures cellular activity instead of simply a change in biomass. T he variations of pH caused by fungal activity were measured by changes in optical densities at 450 nm. The MICs(50) obtained were compared w ith the MICs found by the classical broth macrodilution procedure. In most cases broth micro- and macrodilution MICs were in agreement for a mphotericin B, fluconazole, flucytosine and nystatin.