R. Putti et al., CELL COMPOSITION AND CO-STORED PEPTIDES IN THE ENDOCRINE PANCREAS OF RANA-ARVALIS, European journal of histochemistry, 39(1), 1995, pp. 59-68
The endocrine pancreas of Rana arvalis studied by immunochemistry cons
isted of islets and diffuse endocrine cells. The islets showed a mamma
lian-like arrangement with a central core of B cells and a peripheral
mantle of A/PP cells. A few D and vasoactive intestinal polypeptide ce
lls were also present. Consecutive sections or double-labeling studies
allowed us to detect several regulatory peptides colocalized in the s
ame endocrine cells. The so-called A/PP cells contained subpopulations
of cells showing various types of immunoreactivity and varying degree
s of immunolabeling. Generally, glucagon/pancreatic polypeptide, gluca
gon/pancreatic polypeptide/phe-met-arg-phe-amide, glucagon/pancreatic
polypeptide/peptide tyrosine tyrosine, glucagon/pancreatic polypeptide
/peptide tyrosine tyrosine/phe-met-arg-phe-amide immunoreactivities we
re present in the islets, while peptide tyrosine tyrosine/neuropeptide
tyrosine colocalization was also found in the parenchyma.