V. Lefebvre et al., CONSTRUCTION OF AN INTRASPECIFIC INTEGRATED LINKAGE MAP OF PEPPER USING MOLECULAR MARKERS AND DOUBLED HAPLOID PROGENIES, Genome, 38(1), 1995, pp. 112-121
An integrated molecular linkage map of pepper (Capsicum annuum L.), in
cluding mainly RFLP and RAPD markers, has been constructed by alignmen
t of three intraspecific linkage maps generated by segregating doubled
-haploid progenies. A total of 85 markers covered approximately 820 cM
in 14 linkage groups. Four linkage groups were assigned to 4 chromoso
mes. Two new genes of agronomic interest were located: L controlling h
ypersensitive resistance to TMV and up controlling the erect habit of
the fruits. The C gene controlling the fruit pungency was more precise
ly located. This map is estimated to represent from 36 to 59% of the t
otal pepper genome. An examination of segregation data has revealed se
veral genomic regions with aberrant segregation ratios often favouring
the agronomic big-fruited parents, particularly in crosses involving
the exotic parent CM334, suggesting that these genome regions are subj
ected to selection during the process of doubled-haploid production. T
he suitability of doubled-haploid progenies for mapping projects and t
he differences observed between this intraspecific integrated map with
earlier published interspecific pepper maps are discussed.