BIOCHEMICAL-MECHANISM OF ACTION OF PYRIDOXINE IN THE PREVENTION OF GLYCOLATE INDUCED HYPEROXALURIA IN MALE ALBINO-RATS

Sodium glycolate feeding (100 mg/100 g BW/day) to adult male rats for a period of six weeks resulted in significant hyperoxaluria which was due to enhanced activities of oxalate synthesising enzymes glycolic ac id oxidase (GAG) and glycolic acid dehydrogenase (GAD) in the liver. A dministration of pyridoxine (10 mg/100 g BW/day) along with sodium gly colate resulted in a significant decrease in urinary oxalate as compar ed to glycolate fed rats. Pyridoxine prevented glycolate induced hyper oxaluria by lowering the activities of GAO and GAD. The increased lact ate dehydrogenase (LDH) activity in these animals promotes conversion of glycolate to glyoxylate which may be rapidly transminated to glycin e by pyridoxal phosphate dependent aminotransferases. Pyridoxine admin istration also produced a significant increase in urinary citrate leve ls in glycolate fed rats.