LIQUID-CHROMATOGRAPHIC DETERMINATION OF SALBUTAMOL AND CLENBUTEROL RESIDUES IN SWINE SERUM AND MUSCLE

A simple and rapid method has been developed for determination of resi dual clenbuterol and salbutamol in swine serum and muscle using high-p erformance liquid chromatography. In a Nova-pak C-18 column, the drug peaks were separated by a gradient using two mobile phases [acetonitri le:water (15:85, v/v) and acetonitrile:water (30:70, v/v)] far salbuta mol and clenbuterol, respectively. A photodiode array set at 196 nm fo r salbutamol and 210 nm for clenbuterol, -0.01 to +0.2 AU, ambient tem perature and flow rate of 1.0 ml/min, were used. The retention times w ere 5.4 and 21 min for salbutamol and clenbuterol, respectively. The d etection limits for this method were 0.1 and 0.2 mu l/ml for salbutamo l and clenbuterol, respectively. Average recoveries (0.1, 0.25, 0.5, 1 .0 ppm spiked level) were 86.7 +/- 1.7% for salbutamol; 80.0 +/- 2.0% for clenbuterol spiked in serum; 64.2 +/- 8.9% for salbutamol; 62.9 +/ - 4.2% for clenbuterol spiked in the muscle, respectively. The minimum detectable amount was 0.1 ppm, based on a spiked sample extract.