TESTIS AND SOMATIC XRCC-1 DNA-REPAIR GENE-EXPRESSION

The human XRCC1 gene has been shown to be involved in DNA strand-break repair using the Chinese hamster ovary cell mutant EM9. The purpose o f this study was to characterize the expression of Xrcc-1 to determine if there is tissue-specific expression and to provide a baseline of i nformation for future studies that may involve altering Xrcc-1 express ion in mice. Normal young adult male testis and enriched populations o f pachytene spermatocytes and round spermatids displayed significantly higher levels of Xrcc-1 expression than other mouse tissues, although Xrcc-1 transcripts were found in low abundance in all tested tissues. Cultured mouse cell lines displayed levels of expression similar to m ale germ cells, which is a striking contrast to the levels of expressi on obtained in somatic tissues from the mouse. The relatively high lev els of expression identified in male germ cells indicate Xrcc-1 may ha ve an important role in male germ cell physiology.