P10 GENES OF BOMBYX-MORI NUCLEAR POLYHEDROSIS-VIRUS AND AUTOGRAPHA-CALIFORNICA MULTIPLE NUCLEAR POLYHEDROSIS-VIRUS

EcoR I-P fragment has been cloned from Autographa califormica multiple nuclear polyhedrosis virus (AcMNPV) genomic DNA and used as a probe. 0.5-kb and 1.1-kb fragments including p10 gene from Bombyx mori nuclea r polyhedrosis virus (BmNPV) have been hybridized. The p10 ORF was loc ated in the EcoR I-R fragment. Initiation codon ATG of p10 from BmNPV has been mutated by PCR, and the ATG region became a Bgl II site. A no vel transfer vector pBmAcPV-1 has been constructed using both the p10 5'-flanking region whose initiation codon ATG has been mutated with Bm NPV and the p10 3'-flanking region of AcMNPV. The vector can recombine with not only AcMNPV DNA to express foreign gene in Sf cells, but als o BmNPV DNA to express foreign gene in Bm cells. CAT gene was expresse d at high level in Bm cells under the control of the mutated p10 promo ter of BmNPV.