EFFECT OF CHEMICAL MODIFICATION OF ISOENZYME-A AND ISOENZYME-B FROM C-RUGOSA ON THEIR ACTIVITY AND STABILITY

Authors
CALVO MV PLOU FJ PASTOR E BALLESTEROS A
Citation
Mv. Calvo et al., EFFECT OF CHEMICAL MODIFICATION OF ISOENZYME-A AND ISOENZYME-B FROM C-RUGOSA ON THEIR ACTIVITY AND STABILITY, Biotechnology letters, 17(2), 1995, pp. 171-176
Citations number
18
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
17
Issue
2
Year of publication
1995
Pages
171 - 176
Database
ISI
SICI code
0141-5492(1995)17:2<171:EOCMOI>2.0.ZU;2-B
Abstract
Lipases A and B from C.rugosa have been chemically modified with octan oyl chloride or polyethylene glycol (molecular weight: 750 or 5000). A lthough the hydrolytic activity was diminished, modification gave rise in some cases to preparations with increased synthetic activity in he xane. Furthermore, PEG(5000)-lipases A and B were significantly more s table (up to 20-fold) at alkaline pH values than the native enzymes.