A PROCESS FOR BIOCONVERSION OF CEPHALOSPORIN-C BY RHODOTORULA-GRACILIS D-AMINO-ACID OXIDASE

A process for the production (in a stirred tank reactor) of glutaryl-7 -ACA from cephalosporin C using immobilized D-amino acid oxidase is de scribed. Results so obtained under optimal conditions (1.2 mg coupled enzyme/L, pH 8.5, 2 mM cephalosporin C) point to a system which shows high conversion efficiency and a remarkable operational stability. No exogenous H2O2 is requested to shift the reaction equilibrium toward g lutaryl-7-ACA production, nor any side product is detected. The immobi lized system productivity was 54 g/day/mg of enzyme. This process repr esents the first reported case of a reactor successfully developed wit h a DAAO for bioconversion of cephalosporin C.