SIZE ANALYSIS OF POLY(3-HYDROXYBUTYRIC ACID) GRANULES PRODUCED IN RECOMBINANT ESCHERICHIA-COLI

Authors
MIDDELBERG APJ LEE SY MARTIN J WILLIAMS DRG CHANG HN
Citation
Apj. Middelberg et al., SIZE ANALYSIS OF POLY(3-HYDROXYBUTYRIC ACID) GRANULES PRODUCED IN RECOMBINANT ESCHERICHIA-COLI, Biotechnology letters, 17(2), 1995, pp. 205-210
Citations number
16
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
17
Issue
2
Year of publication
1995
Pages
205 - 210
Database
ISI
SICI code
0141-5492(1995)17:2<205:SAOPAG>2.0.ZU;2-H
Abstract
Size distributions of PHB granules synthesized in recombinant Escheric hia coli are determined by photosedimentation. Mean granule Stokes dia meters are in the range 1.13-1.25 mu m, which is larger than reported values for wild type microorganisms. Treatment with 1.5% hypochlorite and mild centrifugation did not affect granule size distribution. Trea tment with 10% hypochlorite led to a significant reduction in mean dia meter and total PHB.