C. Mascaro et al., MOLECULAR-CLONING AND TISSUE EXPRESSION OF HUMAN MITOCHONDRIAL 3-HYDROXY-3-METHYLGLUTARYL-COA SYNTHASE, Archives of biochemistry and biophysics, 317(2), 1995, pp. 385-390
Mitochondrial 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase
is a constituent of the HMG-CoA pathway responsible for ketone body s
ynthesis and one of its main regulatory points. We report the isolatio
n and characterization of a 2058-bp cDNA from human liver. This cDNA e
ncodes a polypeptide of 508 residues and 56,635 Da. The homology with
previously reported rat mitochondrial and human cytosolic HMG-CoA synt
hases is 88 and 66%, respectively. mRNA levels were high in liver and
colon, low in testis, heart, skeletal muscle, and kidney, and faint in
pancreas. (C) 1995 Academic Press, Inc.