IN-VITRO METHOD FOR MEDICAL RISK ASSESSMENT OF LASER FUMES

Laser processing of different materials may produce toxic fumes. In pr eventive occupational medicine it is necessary to evaluate valid hygie nic standards for work places. The basis for such hygienic standards i s the classification of laser fumes by their fibrogenic, emphysematous , immunological or other harmful potencies in biological assay systems . This paper is part of a European project on laser safety. Our part i n this project is the development of a method for the investigation of lung responses using in vitro cell assays. The appropriate laser fume samples will be supplied by other groups in this European project. In contrast to the cell assays usually used in risk assessment, our meth od is based on isolated target cells in the lung, such as alveolar mac rophages. The test criteria are mediator release, surfactant reactions , release of reactive oxygen species and cell proliferation. As demons trated in the lung response to other dusts (minerals, fibres etc) thes e parameters are medically relevant factors in the pathogenic alveolar dust response. The paper gives basic information about the method usi ng lung cell assays and the results of known substances, in comparison with a dust generated by laser processing.