DETERMINATION OF COMPONENTS IN EYE LOTION BY CAPILLARY ELECTROPHORESIS

Capillary zone electrophoresis (CZE) and micellar electrokinetic chrom atography (MEKC) with sodium dodecyl sulfate (SDS) as an anionic carri er were applied to the analysis of nine principal components of eye lo tion. Simultaneous separation of these components except cyanocobalami n (VB12), which has the same migration time with that of electroosmoti c flow, was achieved by CZE using a 72 cm x 50 mu m i.d. fused silica capillary tube filled with 20 mM borate buffer (pH 10) at 25 kV DC vol tage with UV detection at 200 nm. Under these conditions, the peak of chondroitin sulfate (CS) was overlapped with that of aspartic acid in case the latter was contained in the sample as a co-existing component . However, when pH was decreased to 9, CS was separated from other com ponents and also from aspartic acid, and gave a more shapely peak. VB1 2 could be separated from other components by MEKC with 20 mM berate b uffer (pH 10) containing 40 mM SDS. The applicability to the determina tion of nine principal components of eye lotion is demonstrated.