A SPECTROPHOTOMETRIC ASSAY FOR PHOSPHOLIPASE-D

The preparation of phosphatidyl-p-nitrophenol and its use as a substra te in the non-enzyme based assay for the quantitative evaluation of ph ospholipase D activity is described. The solution of the substrate in Tris buffer pH 8 and Triton X-100 is mixed directly with an aliquot of a fermentation broth or a plant extract and the absorbance of the sol ution at 405 nm is read. The method is rapid, accurate and inexpensive . Application in the pH range 4-9 is possible.