PRODUCTION, ISOLATION AND PARTIAL-PURIFICATION OF XYLANASES FROM AN ASPERGILLUS SP

Citation
P. Khanna et al., PRODUCTION, ISOLATION AND PARTIAL-PURIFICATION OF XYLANASES FROM AN ASPERGILLUS SP, World journal of microbiology & biotechnology, 11(2), 1995, pp. 242-243
Citations number
5
Categorie Soggetti
Biothechnology & Applied Migrobiology
ISSN journal
09593993
Volume
11
Issue
2
Year of publication
1995
Pages
242 - 243
Database
ISI
SICI code
0959-3993(1995)11:2<242:PIAPOX>2.0.ZU;2-4
Abstract
An Aspergillus sp., isolated from a rubbish dump, produced 10.6 IU ml( -1) xylanase activity. Two xylanases were recognized and each was puri fied to homogeneity by two-stage chromatography on DEAE- and CM-Sephar ose. Xylanase I had a pi of 7.2 and an M(r) of 26 kDa whereas xylanase II had a pi of 4.7 and an M(r) of 21 kDa. At 50 degrees C, xylanase I was stable for 2.5 h but xylanase II was only stable for I h.