A tissue culture practical is described which uses vegetable Brassica
seedlings germinated in-vitro and cut up into root, hypocotyl, and cot
yledon explants. These are then cultured on media containing different
levels of phytohormones which induce shoot, root, and callus producti
on in as little as Four to six weeks. The practical is simple and easy
to use and does not require the use of a lamina flow cabinet. Further
developments from the basic schedule are suggested.