QUANTITATIVE THIN-LAYER CHROMATOGRAPHY OF INDOLE ALKALOIDS .2. CATHARANTHINE AND VINDOLINE

A method is described for the densitometric determination of the indol e alkaloids catharanthine and vindoline in plant extract. The alkaloid s were separated from each other and from the rest of the components b y three-fold development with a mobile phase of petroleum ether / ethy l ether / acetone / ethanol (70+10+20+1, v/v/v/v). Catharanthine was s canned at 280 nm and vindoline - at 310 nm. The calibration curves wer e linear in the interval 1 mu g per spot - 5 mu g/per spot. A standard deviation of less than 0.1 mu g per spot and a relative error not exc eeding 3% were found.