SIMPLE HPLC METHOD FOR THE DETERMINATION OF THYMOQUINONE IN BLACK SEED OIL (NIGELLA-SATIVA LINN)

Authors
ABOULENEIN HY ABOUBASHA LI
Citation
Hy. Aboulenein et Li. Aboubasha, SIMPLE HPLC METHOD FOR THE DETERMINATION OF THYMOQUINONE IN BLACK SEED OIL (NIGELLA-SATIVA LINN), Journal of liquid chromatography, 18(5), 1995, pp. 895-902
Citations number
15
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of liquid chromatography → ACNP
ISSN journal
01483919
Volume
18
Issue
5
Year of publication
1995
Pages
895 - 902
Database
ISI
SICI code
0148-3919(1995)18:5<895:SHMFTD>2.0.ZU;2-F
Abstract
A simple and reliable isocratic normal phase HPLC method for the deter mination of thymoquinone in black seed oil (Nigella Sativa Linn, Rancu laceae) is described. After oil extraction with methanol, thymoquinone is analyzed using Econosphere CN column. The mobile phase consists of hexane:2 propanol (99:1 v/v), thymoquinone is monitored by UV detecti on at 295 nm. This method is quite specific and sufficiently sensitive with a lower limit of 5 nmoles/ml, within day and between-day assays showed variation coefficient below 5%.