A SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR DETECTING SULFONAMIDE RESIDUES IN SWINE SERUM AND TISSUES AFTER FLUORESCAMINE DERIVATIZATION
Ce. Tsai et F. Kondo, A SENSITIVE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR DETECTING SULFONAMIDE RESIDUES IN SWINE SERUM AND TISSUES AFTER FLUORESCAMINE DERIVATIZATION, Journal of liquid chromatography, 18(5), 1995, pp. 965-976
A highly sensitive and rapid high-performance liquid chromatographic m
ethod for determining sulfonamides (sulfadiazine, sulfamethazine, sulf
amonomethoxine, sulfamethoxazole and sulfadimethoxine) in swine serum
and tissues is described. The sulfonamides were extracted from the sam
ples, derivatized with fluorescamine, chromatographed on a Nova-Pak C-
18 column using acetonitrile-10 mM potassium phosphate (30:70, v/v) as
the mobile phase and detected spectrofluororimetrically (excitation 3
90 nm, emission 475 nm). The retention times were 7.1 to 18.2 min and
there was no interference from any co-extractives. The detection limit
for each standard sulfonamide solution was 0.1 ng/ml and their calibr
ation curves were linear between 1 and 100 ng/ml. In the presence of s
ulfadiazine as an internal standard, sulfonamide recovery from spiked
serum, muscle, liver and kidney samples (10 ng/ml) was 94.0 +/- 4.7 to
97.3 +/- 5.9%, 58.5 +/- 3.1 to 73.9 +/- 5.7 %, 65.9 +/- 7.1 to 86.9 /- 10.6% and 86.2 +/- 4.0 to 92.8 +/- 6.4% respectively.