A SINGLE-TUBE MULTIPLEX SYSTEM FOR THE SIMULTANEOUS DETECTION OF 10 COMMON CYSTIC-FIBROSIS MUTATIONS

We describe a nonisotopic single tube polymerase chain reaction (PCR) multiplex system that detects 10 of the more common cystic fibrosis (C F) mutations (Delta F508, Delta I507, V520F, G551D, G542X, R553X, R117 H, 621+1G-->T, N1303K, A455E). The use of this method detects approxim ately 90% of the CF alleles in the British population, Five exons of t he CF gene are amplified simultaneously by PCR, followed by an overnig ht triple enzyme restriction digest, and then resolved by high resolut ion gel acrylamide electrophoresis. (C) 1995 Wiley-Liss, Inc.