CLONING OF A FULL-LENGTH CDNA SEQUENCE ENCODING A CDC2-RELATED PROTEIN-KINASE FROM HUMAN ENDOTHELIAL-CELLS

cDNA clones encoding a novel serine/threonine protein kinase were isol ated from human endothelial cell cDNA libraries. The compiled nucleoti de sequence is 1757 base pairs in length and contains an open reading frame encoding a 372 amino acid protein, designated C-2k, with a calcu lated molecular weight of 43 kDa. Sequence analysis indicates that C-2 k contains a conserved protein kinase catalytic domain of 308 residues which exhibits its highest sequence identity of 42% to members of the cdc2 kinase family and contains the structural elements characteristi c to cdc2-like kinases. C-2k may therefore represent a new member of t he cdc2 kinase family. (C) 1995 Academic Press, Inc.