HUMAN PLATELET PHENOLSULFOTRANSFERASES - CDNA CLONING, STABLE EXPRESSION IN V79 CELLS AND IDENTIFICATION OF A NOVEL ALLELIC VARIANT OF THE PHENOL-SULFATING FORM
Al. Jones et al., HUMAN PLATELET PHENOLSULFOTRANSFERASES - CDNA CLONING, STABLE EXPRESSION IN V79 CELLS AND IDENTIFICATION OF A NOVEL ALLELIC VARIANT OF THE PHENOL-SULFATING FORM, Biochemical and biophysical research communications, 208(2), 1995, pp. 855-862
We have isolated cDNA clones encoding phenol- and monoamine-sulfating
phenolsulfotransferases, using the reverse transcription-polymerase ch
ain reaction method with human platelet mRNA as template. These cDNAs
were stably expressed in the Chinese hamster fibroblast cell line V79
and their substrate specificities towards known sulfotransferase isoen
zyme-selective compounds determined. The nucleotide and derived amino
acid sequences of the monoamine-sulfating form were identical to those
previously identified in human liver and brain, but the cDNA for the
human platelet phenol-sulfating form we isolated contained 5 and 2 ami
no acid changes from the two previously published sequences from human
liver and brain, suggesting that we have identified a new allelic var
iant of human phenol-sulfating phenol sulfotransferase. (C) 1995 Acade
mic Press, Inc.