DIRECT EVIDENCE OF A HETEROTRIMERIC COMPLEX OF HUMAN INTERLEUKIN-4 WITH ITS RECEPTORS

Authors
HOFFMAN RC CASTNER BJ GERHART M GIBSON MG RASMUSSEN BD MARCH CJ WEATHERBEE J TSANG M GUSTCHINA A SCHALKHIHI C RESHETNIKOVA L WLODAWER A
Citation
Rc. Hoffman et al., DIRECT EVIDENCE OF A HETEROTRIMERIC COMPLEX OF HUMAN INTERLEUKIN-4 WITH ITS RECEPTORS, Protein science, 4(3), 1995, pp. 382-386
Citations number
41
Categorie Soggetti
Biology
Journal title
Protein science → ACNP
ISSN journal
09618368
Volume
4
Issue
3
Year of publication
1995
Pages
382 - 386
Database
ISI
SICI code
0961-8368(1995)4:3<382:DEOAHC>2.0.ZU;2-9
Abstract
The mode of binding of interleukin-4 (IL-4) to its two known receptors , specific receptor IL-4R and a shared receptor gamma(c), was investig ated using gel filtration and gel electrophoresis. A ternary complex b etween IL-4 and the soluble domains of the two receptors was shown to exist in solution. The association constant between gamma(c) and the s table complex of IL-4/sIL-4R is in the millimolar range, making the te rnary complex a feasible target for crystallization studies.