Phytophthora infestans is able to produce oospores in leaves of potato
and tomato plants after inoculation with a mixture of A1 and A2 matin
g-type isolates. Various conditions for oospore formation were analyse
d. Under controlled conditions, oospores were produced in potato leave
s at temperatures ranging from 5 to 25 degrees C. In leaves of potato
cultivar Bintje incubated at 15 degrees C, oogonia and antheridia were
observed 6 days after inoculation and thick-walled oospores appeared
3-4 days later. In field experiments oospores were found in leaves and
stems of potato cultivars Bintje, Irene and Pimpernel and in leaves,
stems and fruits of tomato cultivar Moneymaker within 2 weeks after in
oculation. A bioassay was developed to test the survival of oospores i
n soil under various conditions. To determine whether late-blight infe
ctions derived from infectious soil were caused by oospores, DNA finge
rprinting was performed. DNA fingerprint probe RG-57 was suitable for
distinguishing asexual progeny from recombinant progeny arising from s
oil-borne oospores. We demonstrated survival of viable, infectious oos
pores of P. infestans in soil during the winter of 1992-93. Oospores w
ere not infectious from soil exposed to temperatures of 40 degrees C o
r higher but in the range 35 degrees C to as low as -80 degrees C for
48 h, oospores survived.