DEFECTIVE SPONTANEOUS AND BACTERIAL LIPOPOLYSACCHARIDE-STIMULATED PRODUCTION OF INTERLEUKIN-1 RECEPTOR ANTAGONIST BY POLYMORPHONUCLEAR NEUTROPHILS OF PATIENTS WITH ACTIVE SYSTEMIC LUPUS-ERYTHEMATOSUS

Interleukin-1 receptor antagonist (IL-1ra) binds competitively to IL-1 receptors but does not transduce the signal which blocks the biologic al activities induced by IL-1. In this study, polymorphonuclear neutro phils (PMN) and mononuclear cells (MNC) from the patients with active systemic lupus erythematosus (SLE) (n = 11), inactive SLE (n = 13) and normal individuals (n = 13) were compared for the IL-1ra producing ca pacity of these cells. PMN and MNC at a concentration of 1 x 10(6) cel ls/ml were incubated with medium alone (spontaneous) or stimulated wit h lipopolysaccharide (LPS, 100 ng/ml) for 24 h. The IL-1ra concentrati on in the supernatants was quantified by ELISA method. Both spontaneou s and LPS-stimulated production of IL-1ra by PMN, but not by MNC, of a ctive SLE were significantly lower than that of inactive SLE or normal groups. Prednisolone (1 and 5 mu g/ml) did not change the production of IL-1ra by normal PMN either spontaneously or LPS-stimulation in in vitro study. Moreover, the IL-1ra producing capacity of PMN in seven a ctive SLE on admission and after intensive immunosuppressive treatment was measured. These results suggest that the defective IL-1ra product ion by SLE-PMN is relevant to disease activity and may be regarded as a new indicator of disease activity in patients with active SLE.