PREPARATION OF AFFINITY SORBENTS AND ISOLATION OF INDIVIDUAL CHITINASES FROM A CRUDE SUPERNATANT PRODUCED BY STREPTOMYCES-KURSSANOVII BY A ONE-STEP AFFINITY-CHROMATOGRAPHIC SYSTEM
Av. Ilyina et al., PREPARATION OF AFFINITY SORBENTS AND ISOLATION OF INDIVIDUAL CHITINASES FROM A CRUDE SUPERNATANT PRODUCED BY STREPTOMYCES-KURSSANOVII BY A ONE-STEP AFFINITY-CHROMATOGRAPHIC SYSTEM, Biotechnology and applied biochemistry, 21, 1995, pp. 139-148
The preparation of two affinity-chromatography sorbents based on cross
-linked chitin are described. Both sorbents retained selectively one o
f the four extracellular chitinases in the culture supernatant produce
d by Streptomyces kurssanovii. Chitinases with molecular masses of 42
kDa and 26 kDa were isolated in homogeneous form using one-step affini
ty-chromatography procedures involving either a fully N-acetylated or
a partially N-acetylated crosslinked chitin-type sorbent. Two other ch
itinases were not selectively bound by the sorbents and, therefore, we
re not isolated in a homogeneous form. The affinity sorbents were show
n to be stable over the period of separation and could be used repeate
dly.