R. Tyagi et Mn. Gupta, IMMOBILIZATION OF ASPERGILLUS-NIGER XYLANASE ON MAGNETIC LATEX BEADS, Biotechnology and applied biochemistry, 21, 1995, pp. 217-222
Xylanase from Pectinex 3XL was purified 70-fold by precipitation with
an enteric polymer, Eudragit S-100. The purified xylanase was immobili
zed on magnetic latex beads via carbodi-imide coupling. The immobilize
d preparation showed 80% of the total activity bound to the beads. The
pH optimum remained unchanged at 6.0 and the K-m increased from 0.25
g/100 ml (free enzyme) to 0.39 g/100 mi on immobilization. Immobilizat
ion resulted in significant thermal stability at 60 degrees C. The tim
e course of hydrolysis of xylan at 60 degrees C by free enzyme as well
as immobilized enzyme was also studied.